Describe your analyte to start
Set the molecule class, LogP, pKa, MW, solubility, and detection on the left, then generate a starting column, mobile phase, gradient, operating parameters, and troubleshooting hints tuned to your compound.
A free tool by Mirela Gorjanu · Practical Pharma Science™
Starting Method Protocol
01Stationary Phase (Column)
Chemistry
Dimensions
Particle
02Mobile Phase Strategy
03Initial Gradient Program
| Time (min) | % B | Step |
|---|---|---|
Gradient profile (%B vs time)
04Operating Parameters
Flow rate
Column temp.
Injection
Detection
Sample diluent
05Method Development & Troubleshooting Hints
Screening starting conditions generated from your inputs · optimise empirically (selectivity, robustness, system suitability) before use. This is a method-planning aid, not a validated computerised system, and it holds no controlled GxP record.
Generated with the free HPLC Method Development & Optimizer ·
Produced by Mirela Gorjanu · Practical Pharma Science™ ·
You are using the free tool
This gives you one compound and one starting method.
It cannot tell you whether your peaks will separate from each other, and its conditions are generic rather than calibrated to your instrument. Those are the two things that actually cost you weeks at the bench. HPLC Assist Pro™ is the tool that does them.
Free tool — this page
- One analyte at a time
- Generic starting column & gradient
- No resolution between peaks
- Nothing saved between visits
HPLC Assist Pro™
- Your whole mixture, simulated chromatogram and worst-pair resolution
- 14-column library — retention, plates and pressure change with the column
- Auto-Optimize searches column × %B × pH for the best separation
- Two-run calibration fits the retention model to your system
- Resolution design space, pH scouting, gradient & peak capacity
- SST checker (USP ⟨621⟩ / Ph. Eur. 2.2.46 / JP), ICH Q2(R2) planner
- Branded PDF report, saved methods and projects, PubChem lookup
Free tool by Mirela Gorjanu · Practical Pharma Science™ — HPLC & LC-MS courses, guides, templates and tools for working analysts.